mcherry rab4 (Addgene inc)
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Mcherry Rab4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcherry+rab4/mCherry-Rab4a-7+(Plasmid+%2355125)/pmc12058456-67-25-26
Average 93 stars, based on 13 article reviews
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Clone Assay:Article Title: Boc Acts via Numb as a Shh-Dependent Endocytic Platform for Ptch1 Internalization and Shh-Mediated Axon Guidance. Article Snippet: Monodansyl cadaverin (MDC) was used at 100 mM (Sigma). .. Boc-GFP and Numb-Myc were cloned in our labs (Okada et al., 2006; Ramamurthy et al., 2014). mRFP-Rab5 and mRFP-Rab7 were a gift from Ari Helenius (Vonderheit and Helenius, 2005), Addgene plasmid #14437 and #14436, respectively), DsRed-rab11 WT was a gift from Richard Pagano (Choudhury et al., 2002), Addgene plasmid #12679), Plasmid Preparation:Article Title: Boc Acts via Numb as a Shh-Dependent Endocytic Platform for Ptch1 Internalization and Shh-Mediated Axon Guidance. Article Snippet: Monodansyl cadaverin (MDC) was used at 100 mM (Sigma). .. Boc-GFP and Numb-Myc were cloned in our labs (Okada et al., 2006; Ramamurthy et al., 2014). mRFP-Rab5 and mRFP-Rab7 were a gift from Ari Helenius (Vonderheit and Helenius, 2005), Addgene plasmid #14437 and #14436, respectively), DsRed-rab11 WT was a gift from Richard Pagano (Choudhury et al., 2002), Addgene plasmid #12679), Article Title: Hyperactivation of RAB5 disrupts the endosomal Rab cascade leading to endolysosomal dysregulation in Down syndrome: A necessary role for increased APP gene dose Article Snippet: GFP‐RAB5, mCherry‐RAB7, DsRed‐RAB11, and mCherry‐RAB4 were subcloned into pLenti CMV GFP Puro (Addgene plasmid #17448). .. GFP‐RAB5 was kindly provided by Dr. Marino Zerial (Max Planck Institute of Molecular Cell Biology and Genetics). mCherry‐RAB7 was obtained from Dr. Chengbiao Wu (UCSD). Article Title: Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling Article Snippet: For amino acid starvation, cells were incubated in amino acid‐free DMEM for 4 h. The following inhibitors were used: Dynasore (30 μM, Sigma), monensin (100 μM, Sigma), bafilomycin A1 (20 nM, Cell Signaling Technologies), momelotinib (5 μM, Selleck Chemicals) and MRT68601 (100 μM, Tocris). .. For expression in XFM/ tv‐a glial cells, the following plasmids were used: lentiCas9‐Blast (gift from Feng Zhang, Addgene plasmid #52962), RCAS‐Y vector (gift from William Pavan, Addgene #11478), MSCV‐EGFRvIII (gift from Alonzo Ross, Addgene #20737), YFP‐Galectin‐3, YFP‐Galectin‐8 or YFP‐Galectin‐9 (kind gifts from Felix Randow: Thurston et al , 2012), Derivative Assay:Article Title: Boc Acts via Numb as a Shh-Dependent Endocytic Platform for Ptch1 Internalization and Shh-Mediated Axon Guidance. Article Snippet: Monodansyl cadaverin (MDC) was used at 100 mM (Sigma). .. Boc-GFP and Numb-Myc were cloned in our labs (Okada et al., 2006; Ramamurthy et al., 2014). mRFP-Rab5 and mRFP-Rab7 were a gift from Ari Helenius (Vonderheit and Helenius, 2005), Addgene plasmid #14437 and #14436, respectively), DsRed-rab11 WT was a gift from Richard Pagano (Choudhury et al., 2002), Addgene plasmid #12679), Expressing:Article Title: Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling Article Snippet: For amino acid starvation, cells were incubated in amino acid‐free DMEM for 4 h. The following inhibitors were used: Dynasore (30 μM, Sigma), monensin (100 μM, Sigma), bafilomycin A1 (20 nM, Cell Signaling Technologies), momelotinib (5 μM, Selleck Chemicals) and MRT68601 (100 μM, Tocris). .. For expression in XFM/ tv‐a glial cells, the following plasmids were used: lentiCas9‐Blast (gift from Feng Zhang, Addgene plasmid #52962), RCAS‐Y vector (gift from William Pavan, Addgene #11478), MSCV‐EGFRvIII (gift from Alonzo Ross, Addgene #20737), YFP‐Galectin‐3, YFP‐Galectin‐8 or YFP‐Galectin‐9 (kind gifts from Felix Randow: Thurston et al , 2012), |
