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mcherry rab4  (Addgene inc)


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    Structured Review

    Addgene inc mcherry rab4
    Mcherry Rab4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+rab4/mCherry-Rab4a-7+(Plasmid+%2355125)/pmc12058456-67-25-26
    Average 93 stars, based on 13 article reviews
    mcherry rab4 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Boc Acts via Numb as a Shh-Dependent Endocytic Platform for Ptch1 Internalization and Shh-Mediated Axon Guidance.
    Article Snippet: Monodansyl cadaverin (MDC) was used at 100 mM (Sigma). .. Boc-GFP and Numb-Myc were cloned in our labs (Okada et al., 2006; Ramamurthy et al., 2014). mRFP-Rab5 and mRFP-Rab7 were a gift from Ari Helenius (Vonderheit and Helenius, 2005), Addgene plasmid #14437 and #14436, respectively), DsRed-rab11 WT was a gift from Richard Pagano (Choudhury et al., 2002), Addgene plasmid #12679), mCherry-Rab4 (gift fromMichael Davidson, Addgene plasmid #55125). pGex4T-mBocWT, pGex4T-mBocD1 and pGex4T-mBocD2 were derived from pEGFP-mBoc (Okada et al., 2006). ..

    Plasmid Preparation:

    Article Title: Boc Acts via Numb as a Shh-Dependent Endocytic Platform for Ptch1 Internalization and Shh-Mediated Axon Guidance.
    Article Snippet: Monodansyl cadaverin (MDC) was used at 100 mM (Sigma). .. Boc-GFP and Numb-Myc were cloned in our labs (Okada et al., 2006; Ramamurthy et al., 2014). mRFP-Rab5 and mRFP-Rab7 were a gift from Ari Helenius (Vonderheit and Helenius, 2005), Addgene plasmid #14437 and #14436, respectively), DsRed-rab11 WT was a gift from Richard Pagano (Choudhury et al., 2002), Addgene plasmid #12679), mCherry-Rab4 (gift fromMichael Davidson, Addgene plasmid #55125). pGex4T-mBocWT, pGex4T-mBocD1 and pGex4T-mBocD2 were derived from pEGFP-mBoc (Okada et al., 2006). ..

    Article Title: Hyperactivation of RAB5 disrupts the endosomal Rab cascade leading to endolysosomal dysregulation in Down syndrome: A necessary role for increased APP gene dose
    Article Snippet: GFP‐RAB5, mCherry‐RAB7, DsRed‐RAB11, and mCherry‐RAB4 were subcloned into pLenti CMV GFP Puro (Addgene plasmid #17448). .. GFP‐RAB5 was kindly provided by Dr. Marino Zerial (Max Planck Institute of Molecular Cell Biology and Genetics). mCherry‐RAB7 was obtained from Dr. Chengbiao Wu (UCSD). mCherry‐RAB4 (Addgene plasmid #55125) and DsRed‐RAB11 (Addgene plasmid #12679) were sourced from Addgene. ..

    Article Title: Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling
    Article Snippet: For amino acid starvation, cells were incubated in amino acid‐free DMEM for 4 h. The following inhibitors were used: Dynasore (30 μM, Sigma), monensin (100 μM, Sigma), bafilomycin A1 (20 nM, Cell Signaling Technologies), momelotinib (5 μM, Selleck Chemicals) and MRT68601 (100 μM, Tocris). .. For expression in XFM/ tv‐a glial cells, the following plasmids were used: lentiCas9‐Blast (gift from Feng Zhang, Addgene plasmid #52962), RCAS‐Y vector (gift from William Pavan, Addgene #11478), MSCV‐EGFRvIII (gift from Alonzo Ross, Addgene #20737), YFP‐Galectin‐3, YFP‐Galectin‐8 or YFP‐Galectin‐9 (kind gifts from Felix Randow: Thurston et al , 2012), mCherry‐Rab4 (gift from Michael Davidson, Addgene #55125), mCherry‐Rab5 and mCherry‐Rab11 (gift from Michael Davidson, Addgene #55124). .. For the generation of the RCAS‐shRNA vectors, shRNA sequences were initially cloned into pSUPER.retro vectors (OligoEngine, VEC‐PRT‐0002) .

    Derivative Assay:

    Article Title: Boc Acts via Numb as a Shh-Dependent Endocytic Platform for Ptch1 Internalization and Shh-Mediated Axon Guidance.
    Article Snippet: Monodansyl cadaverin (MDC) was used at 100 mM (Sigma). .. Boc-GFP and Numb-Myc were cloned in our labs (Okada et al., 2006; Ramamurthy et al., 2014). mRFP-Rab5 and mRFP-Rab7 were a gift from Ari Helenius (Vonderheit and Helenius, 2005), Addgene plasmid #14437 and #14436, respectively), DsRed-rab11 WT was a gift from Richard Pagano (Choudhury et al., 2002), Addgene plasmid #12679), mCherry-Rab4 (gift fromMichael Davidson, Addgene plasmid #55125). pGex4T-mBocWT, pGex4T-mBocD1 and pGex4T-mBocD2 were derived from pEGFP-mBoc (Okada et al., 2006). ..

    Expressing:

    Article Title: Targeting of early endosomes by autophagy facilitates EGFR recycling and signalling
    Article Snippet: For amino acid starvation, cells were incubated in amino acid‐free DMEM for 4 h. The following inhibitors were used: Dynasore (30 μM, Sigma), monensin (100 μM, Sigma), bafilomycin A1 (20 nM, Cell Signaling Technologies), momelotinib (5 μM, Selleck Chemicals) and MRT68601 (100 μM, Tocris). .. For expression in XFM/ tv‐a glial cells, the following plasmids were used: lentiCas9‐Blast (gift from Feng Zhang, Addgene plasmid #52962), RCAS‐Y vector (gift from William Pavan, Addgene #11478), MSCV‐EGFRvIII (gift from Alonzo Ross, Addgene #20737), YFP‐Galectin‐3, YFP‐Galectin‐8 or YFP‐Galectin‐9 (kind gifts from Felix Randow: Thurston et al , 2012), mCherry‐Rab4 (gift from Michael Davidson, Addgene #55125), mCherry‐Rab5 and mCherry‐Rab11 (gift from Michael Davidson, Addgene #55124). .. For the generation of the RCAS‐shRNA vectors, shRNA sequences were initially cloned into pSUPER.retro vectors (OligoEngine, VEC‐PRT‐0002) .



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    WDFY2 localization in the endocytic pathway. a SIM image showing representative WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (gray) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEA1-labeled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 µm, insets 1 µm. Representative image of 14 cells. b SIM images showing the localization of GFP-WDFY2 in relation to mCherry-RAB5, <t>mCherry-RAB4,</t> RAB7 or mCherry-RAB11. Shown are representative images of 12(RAB5), 13(RAB4), 14(RAB7, RAB11) cells. Scale bar: 1 µm, insets: 0.5 µm. c Colocalization using Manders’ colocalization coefficient (MCC) analysis of hTERT-RPE1 cells stably expressing GFP-WDFY2 shows overlap between WDFY2 and RAB4 and RAB5, but only moderate overlap with RAB7 and RAB11. n = 3 experiments (Rab4, Rab5, and Rab11) and n = 4 (Rab7) with 70 cells per condition. Shown are individual experiments and the mean ± 95% CI. d Confocal image showing localization of endogenously-tagged WDFY2 (green) and EEA1 (red). Scale bar: 10 µm, insets: 1 µm. Representative image from 14 cells. Source data are provided as a Source Data file
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    Average 93 stars, based on 1 article reviews
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    WDFY2 localization in the endocytic pathway. a SIM image showing representative WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (gray) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEA1-labeled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 µm, insets 1 µm. Representative image of 14 cells. b SIM images showing the localization of GFP-WDFY2 in relation to mCherry-RAB5, <t>mCherry-RAB4,</t> RAB7 or mCherry-RAB11. Shown are representative images of 12(RAB5), 13(RAB4), 14(RAB7, RAB11) cells. Scale bar: 1 µm, insets: 0.5 µm. c Colocalization using Manders’ colocalization coefficient (MCC) analysis of hTERT-RPE1 cells stably expressing GFP-WDFY2 shows overlap between WDFY2 and RAB4 and RAB5, but only moderate overlap with RAB7 and RAB11. n = 3 experiments (Rab4, Rab5, and Rab11) and n = 4 (Rab7) with 70 cells per condition. Shown are individual experiments and the mean ± 95% CI. d Confocal image showing localization of endogenously-tagged WDFY2 (green) and EEA1 (red). Scale bar: 10 µm, insets: 1 µm. Representative image from 14 cells. Source data are provided as a Source Data file
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    Image Search Results


    WDFY2 localization in the endocytic pathway. a SIM image showing representative WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (gray) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEA1-labeled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 µm, insets 1 µm. Representative image of 14 cells. b SIM images showing the localization of GFP-WDFY2 in relation to mCherry-RAB5, mCherry-RAB4, RAB7 or mCherry-RAB11. Shown are representative images of 12(RAB5), 13(RAB4), 14(RAB7, RAB11) cells. Scale bar: 1 µm, insets: 0.5 µm. c Colocalization using Manders’ colocalization coefficient (MCC) analysis of hTERT-RPE1 cells stably expressing GFP-WDFY2 shows overlap between WDFY2 and RAB4 and RAB5, but only moderate overlap with RAB7 and RAB11. n = 3 experiments (Rab4, Rab5, and Rab11) and n = 4 (Rab7) with 70 cells per condition. Shown are individual experiments and the mean ± 95% CI. d Confocal image showing localization of endogenously-tagged WDFY2 (green) and EEA1 (red). Scale bar: 10 µm, insets: 1 µm. Representative image from 14 cells. Source data are provided as a Source Data file

    Journal: Nature Communications

    Article Title: WDFY2 restrains matrix metalloproteinase secretion and cell invasion by controlling VAMP3-dependent recycling

    doi: 10.1038/s41467-019-10794-w

    Figure Lengend Snippet: WDFY2 localization in the endocytic pathway. a SIM image showing representative WDFY2 localization in relation to different markers of the early endocytic pathway. APPL1 (gray) was used as a marker for early vesicles and EEA1 (red) marks early endosome. GFP-WDFY2 (green) localizes to an independent vesicle pool (inset 2) and to subdomains on EEA1-labeled endosomes (inset 3). There is only limited overlap with APPL1 endosomes (inset 1). Scale bar: 10 µm, insets 1 µm. Representative image of 14 cells. b SIM images showing the localization of GFP-WDFY2 in relation to mCherry-RAB5, mCherry-RAB4, RAB7 or mCherry-RAB11. Shown are representative images of 12(RAB5), 13(RAB4), 14(RAB7, RAB11) cells. Scale bar: 1 µm, insets: 0.5 µm. c Colocalization using Manders’ colocalization coefficient (MCC) analysis of hTERT-RPE1 cells stably expressing GFP-WDFY2 shows overlap between WDFY2 and RAB4 and RAB5, but only moderate overlap with RAB7 and RAB11. n = 3 experiments (Rab4, Rab5, and Rab11) and n = 4 (Rab7) with 70 cells per condition. Shown are individual experiments and the mean ± 95% CI. d Confocal image showing localization of endogenously-tagged WDFY2 (green) and EEA1 (red). Scale bar: 10 µm, insets: 1 µm. Representative image from 14 cells. Source data are provided as a Source Data file

    Article Snippet: The following plasmids were obtained from Addgene: pmCherry-RAB4 (55125) and pmCherry-WASH1-C-18 (55162) were a gift from Michael Davidson, pEGFP-VAMP3 (42310) was a gift from Thierry Galli . pmCherry-CORTACTIN (27676) and CORONIN1B-mCherry (27694) were a gift from Christien Merrifield . pX458 (48138) was a gift from Feng Zhang .

    Techniques: Marker, Labeling, Stable Transfection, Expressing

    WDFY2 controls MT1-MMP trafficking. a Confocal images showing localization of MT1-MMP and VAMP3 to GFP-WDFY2-positive endosome. Representative image from 10 cells. Scale bar: 10 µm, inset: 1 µm. b Confocal images showing localization of MT1-MMP and RAB4 to GFP-WDFY2-positive endosome. Representative image from 10 cells. Scale bar: 10 µm, inset: 1 µm. c TIRF micrograph of WT and WDFY2(−/−) cells transfected with pHluorin-MT1-MMP. Individual exocytic events (summed over two minutes) are indicated with a red circle. Representative image from 30 observed cells per condition. Scale bar: 10 µm, d Quantification of pHluorin-MT1-MMP exocytosis in WT and WDFY2(−/−) cells. Shown are events from three experiments, 10 cells per experiments per condition. Exocytosis events per minute are shown normalized to cell area. Student’s unpaired t test, p = 0.0026. Shown are the median, quartiles (boxes), and 1.5 times the interquartile range (whiskers). * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not statistically significant. e Confocal micrographs of RPE1 (WT) and RPE1 cells stably expressing GFP-WDFY2. Cells overexpressing WDFY2 accumulates MT1-MMP in endosome positive for WDFY2. Scale bar: 10 µm, insets: 5 µm. f Quantification of MT1-MMP spots based on high-content microscopy show a significant increase in total intensity of MT1-MMP in hTERT-RPE1 GFP-WDFY2 cells when compared to hTERT-RPE1 parental cells. Shown are quantifications from three independent experiments, cells in total: hTERT-RPE1: 3570, hTERT-RPE1 GFP-WDFY2: 5250. Plotted are individual experiments and the mean ± 95% CI. p = 0.02007. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not statistically significant. g Quantification of pHlourin-MT1-MMP exocytosis in WDFY2(−/−) cells treated with siControl or siVAMP3. Shown are events from three experiments, ten cells per experiments per condition. Exocytosis events per minute are shown normalized to cell area. Student’s unpaired t-test, p = 0.0149. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not statistically significant. Source data are provided as a Source Data file

    Journal: Nature Communications

    Article Title: WDFY2 restrains matrix metalloproteinase secretion and cell invasion by controlling VAMP3-dependent recycling

    doi: 10.1038/s41467-019-10794-w

    Figure Lengend Snippet: WDFY2 controls MT1-MMP trafficking. a Confocal images showing localization of MT1-MMP and VAMP3 to GFP-WDFY2-positive endosome. Representative image from 10 cells. Scale bar: 10 µm, inset: 1 µm. b Confocal images showing localization of MT1-MMP and RAB4 to GFP-WDFY2-positive endosome. Representative image from 10 cells. Scale bar: 10 µm, inset: 1 µm. c TIRF micrograph of WT and WDFY2(−/−) cells transfected with pHluorin-MT1-MMP. Individual exocytic events (summed over two minutes) are indicated with a red circle. Representative image from 30 observed cells per condition. Scale bar: 10 µm, d Quantification of pHluorin-MT1-MMP exocytosis in WT and WDFY2(−/−) cells. Shown are events from three experiments, 10 cells per experiments per condition. Exocytosis events per minute are shown normalized to cell area. Student’s unpaired t test, p = 0.0026. Shown are the median, quartiles (boxes), and 1.5 times the interquartile range (whiskers). * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not statistically significant. e Confocal micrographs of RPE1 (WT) and RPE1 cells stably expressing GFP-WDFY2. Cells overexpressing WDFY2 accumulates MT1-MMP in endosome positive for WDFY2. Scale bar: 10 µm, insets: 5 µm. f Quantification of MT1-MMP spots based on high-content microscopy show a significant increase in total intensity of MT1-MMP in hTERT-RPE1 GFP-WDFY2 cells when compared to hTERT-RPE1 parental cells. Shown are quantifications from three independent experiments, cells in total: hTERT-RPE1: 3570, hTERT-RPE1 GFP-WDFY2: 5250. Plotted are individual experiments and the mean ± 95% CI. p = 0.02007. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not statistically significant. g Quantification of pHlourin-MT1-MMP exocytosis in WDFY2(−/−) cells treated with siControl or siVAMP3. Shown are events from three experiments, ten cells per experiments per condition. Exocytosis events per minute are shown normalized to cell area. Student’s unpaired t-test, p = 0.0149. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not statistically significant. Source data are provided as a Source Data file

    Article Snippet: The following plasmids were obtained from Addgene: pmCherry-RAB4 (55125) and pmCherry-WASH1-C-18 (55162) were a gift from Michael Davidson, pEGFP-VAMP3 (42310) was a gift from Thierry Galli . pmCherry-CORTACTIN (27676) and CORONIN1B-mCherry (27694) were a gift from Christien Merrifield . pX458 (48138) was a gift from Feng Zhang .

    Techniques: Transfection, Stable Transfection, Expressing, Microscopy